Table 1.
Primary antibodies and IB4 used for IHC in this study.
| Antibodya | Host | Supplier/Catalog#c | Dilution |
|---|---|---|---|
| GINIP (Kiaa1045)b | Goat polyclonal | SCB/sc-247284 | 1:100 |
| Tubb3 | Mouse monoclonal | SCB/sc-80016 | 1:400 |
| NF200 | Mouse monoclonal | Sigma-Aldrich/MAB5256 | 1:1000 |
| CGRP | Mouse monoclonal | SCB/sc-57053 | 1:500 |
| 594-conjugated IB4 | ThermoFisher/I21413 | 5 µg/ml | |
| TRPV1 | Guinea pig polyclonal | Neuromics/GP14100 | 1:100 |
| NaV1.7 | Rabbit polyclonal | Alomone Labs/ASC-008 | 1:100 |
| CaV2.2α1b | Rabbit polyclonal | Atlas Antibodies/HPA044347 | 1:100 |
| CaV3.2α1b | Rabbit polyclonal | Alomone Labs/ACC-025 | 1:100 |
| GABAB receptor 1 | Rabbit polyclonal | Alomone Labs/AGB-001 | 1:50 |
| GABAB receptor 2 | Rabbit polyclonal | Alomone Labs/AGB002 | 1:50 |
| Gαi1 | Rabbit polyclonal | SCB/sc-391 | 1:100 |
| Gαo | Rabbit polyclonal | SCB/sc-387 | 1:100 |
| Trek2 | Rabbit polyclonal | Alomone Labs/APC055 | 1:100 |
| TrkA | Rabbit polyclonal | SCB/sc-118 | 1:200 |
GINIP: Gαi-interacting protein.
aAntibody abbreviations: Tubb3: β3-Tubulin; NF200: Neurofilament 200; CGRP: Calcitonin gene related peptide; Gαi1: G protein G(i) subunit alpha-1; Gαo: G protein G(o) subunit; GBR1: GABAB receptor 1; GBR2: GABAB receptor 2; Trpv1: Vanilloid receptor 1; NaV1.7: Voltage-gated sodium channel 1.7; CaV2.2: VGCC, N type, alpha 1B subunit; CaV3.2: VGCC, T type, alpha 1B subunit; TrkA: Tyrosine kinase receptor type A; Trek2: K channel, subfamily K, member 10.
bIt is an affinity purified goat polyclonal antibody raised against a peptide mapping near the N-terminus of GINIP of human origin.
cSCB, Santa Cruz Biotechnology, Santa Cruz, CA; Sigma–Aldrich, St. Louis, MO; ThermoFisher, Waltham, MA; Neuromics, Edina, MN; Alomone Labs, Jerusalem, Israel; Atlas Antibodies, Stockholm, Sweden.