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ChIP assay for Grm2 and acetylated histone 3. (a) PCR primers designed to cover the proximal transcriptional regulatory region shown schematically (sequences shown in Table 1). (b) PCR amplicons of four regions of the Grm2 promoter were quantified and normalized to relevant input and a desert region. n = 6 for each group. *p < 0.05, **p < 0.01 compared with DMSO pretreatment group for amplicons 1–4 for H3K9Ac or H3K18Ac. TSS: transcription start site.
