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. 2016 Jul 21;11(7):e0159460. doi: 10.1371/journal.pone.0159460

Fig 3. Slug regulates SMC proliferation and migration rate.

Fig 3

A, RT-PCR and inmunofluorescence analysis showed that Slug inhibition promoted downregulation of Ki-67 expression, consistent with decreased S phase of cell cycle analyzed by flow cytometry (B). C, Slug overexpression increases SMC proliferation analyzed by Ki67 expression. F and G, Cell migration was measured by wound-healing assay and expressed as the percentage (%) of SMC migrating/time. Slug knockdown cells display lower migration rates than control cells (F), while Slug-overexpressing SMC exhibit higher migration rate than control cells (G). Data are expressed as the mean ± SEM of four independent experiments performed in duplicate. *p < 0.05 by paired t-test.