Fig 4. Slug regulates both TNFα-induced dedifferentiation and TGFβ1-induced differentiation.

A, The increase of KLF4 and the decrease of SMC marker genes induced by TNFα treatment is prevented by Slug knockdown measured by RT-PCR B-C, TGFβ1-induced increase of SMC markers is dampened in Slug overexpressing cells as observed by RT-PCR (B) and immunofluorescence (C). Data are expressed as mean ± SEM of three independent experiments performed in duplicate. *p < 0.05 by paired t-test.