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. 2016 Mar 15;12:1744806916636387. doi: 10.1177/1744806916636387

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Neurochemical phenotype of lumbar DRG and characterization of articular and cutaneous neuron neurochemical composition. (a–d), example micrographs showing a bright field image of a lumbar DRG section (a), white asterisk shows a neuron that is peptidergic (CGRP positive) (b) and contains RetroBeads (c), black asterisks denotes neurons that are CGRP positive but do not contain RetroBeads, and (d) shows the merged image. (e and f) Percentage of lumbar DRG neurons (combined analysis of L2–L5) that colocalize RetroBeads with different neurochemical markers following injection of retrograde tracer to articular (e) or cutaneous (f) sites (n = 4 animals in each condition). Numbers in brackets refer to the number of RetroBeads labeled neurons upon which this analysis is based. *p < 0.05, **p < 0.01 (one-way ANOVA and Tukey’s post hoc test). DRG: dorsal root ganglia; CGRP: calcitonin gene-related peptide; ANOVA: analysis of variance.