Figure 4.

Acute effects of ML on capsaicin responses in hDRG neurons. Brightfield image of a human DRG neuron (a), showing increased fura 2 ratio in response to 200 nM capsaicin and the corresponding trace (b), followed by complete inhibition of 1 mol/L capsaicin response in the presence of 1.3 mol/L ML (c). The acute inhibitory effect of ML was reversible following washout, and the capsaicin response was restored (d). Graph showing percent inhibition of responses to 1 M capsaicin dose dependently inhibited in the presence of 260 nmol/L ML (**p = 0.003) and 1.3 mol/L ML**p = 0.004 (e).