Figure 5.

Acute effects of ML on capsaicin responses in rDRG neurons. Trace showing increased fura2 ratio in response to 200 nM capsaicin (a). Near complete inhibition was observed in the presence of ML 300 nmol/L (b). The acute inhibitory effect of ML was reversed following a 30-min rest period after washout, and robust responses to capsaicin and ionomycin were observed (c). Graph showing ML dose-related inhibition of capsaicin responses following acute incubation with ML (d). Graph showing inhibition of capsaicin responses by 100 nmol/L ML, which were abolished in the presence of cyclosporine and IBMX (e). *p < 0.05, **p < 0.01, ***p < 0.001.