Fig 1.

Sevoflurane reduces A) filopodia and B) thin spine length in DIV7 immature mouse hippocampal neurons. (A) 3% sevoflurane for four hours reduced mean filopodia length in hippocampal neurons immediately after the exposure. (B) 3% sevoflurane for four hours reduced mean thin spine length in hippocampal neurons immediately after the exposure. (**: the difference between sevoflurane-exposed and unexposed neurons.) Representative fluorescent images of (phalloidin-stained) F-actin containing, control or sevoflurane-exposed dendritic branches show acute length-shortening of A) filopodia or B) thin spines following sevoflurane treatment. N = 167 protrusions, similar results were obtained in three experiments. Calibration bars are 5 microns (A) and 0.5 microns (B) in length.