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. 2016 Jul 21;11(7):e0159637. doi: 10.1371/journal.pone.0159637

Fig 5. The Rho kinase inhibitor, Y27632, prevents sevoflurane-induced filopodial shortening in DIV7 immature hippocampal neurons.

Fig 5

(A) 3% sevoflurane for four hours on DIV7 caused length-shortening in filopodia (solid bars) compared to control unexposed neurons (open bars). The length-shortening was completely prevented by co-incubating sevoflurane-exposed neurons with the selective Rho kinase inhibitor Y27632 (diagonal bars). Y 27632 alone (gray bars) increased filopodia length compared to control neurons. (**: the difference between sevoflurane-exposed and unexposed neurons; ^^ or the difference between sevoflurane-exposed in the presence or absence of Y27632; ## or the difference between control neurons in the presence or absence of Y27632. There was no significant interaction between Y27632 and sevoflurane. N = 318 protrusions, similar results were obtained in two experiments. Representative phalloidin images in (B) control, (C) sevoflurane-exposed, (D) Y27632-treated, or (E) Y2632 plus sevoflurane-exposed neurons. Calibration bars (B-E) are 5 microns in length.