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. 2016 Jul 21;11(7):e0159550. doi: 10.1371/journal.pone.0159550

Fig 2. Liver gene expression of LRP-1, SREBP-2 and IDOL in ovariectomized rats treated with rosuvastatin.

Fig 2

Liver mRNA (A) and protein abundance (B and D) of low-density lipoprotein receptor-related protein 1 (LRP-1) along with mRNA expression (F) of sterol regulatory element binding protein 2 (SREBP-2) were reduced in ovariectomized (Ovx) rats either in sedentary (Sed) and trained (Tr) states and increased with rosuvastatin (Ros) treatment, whereas liver mRNA (G) of inducible degrader of the LDLR (IDOL) was not changed in Ovx rats but was increased by Ros treatment. Values for mRNA as determined by quantitative polymerase chain reaction are expressed as a ratio of the control value (1.0) and are mean ± SD (standard deviation) with n = 8–10 rats per group. Hprt1 (hypoxanthine phosphoribosyltransferase 1) and b-actin were used as endogenous controls. *p < 0.05; ** p < 0.01; *** p < 0.001 significantly different from Sham-Sed-Sal. §p < 0.05; §§p < 0.01 significantly different from respective Saline (Sal) group. Protein abundance in liver of rats were determined by Western blot analysis and normalized to those of b-actin. Quantification (C) of proteins bands in (B) with n = 4 rats/group. Quantification (E) of proteins bands in (D) with n = 2 rats/group. The % changes indicated (E) are in comparison to the Sham-Sed-Sal group.