Fig 6. Chloride involved in the migration of hGFs.

A) Scratch-test assay. B) Remaining wound area was normalized with the time point 0 h. C) NaCl, KCl rinsing did not alter the proliferation of hGFs by MTT assay while NaH₂PO₄, KH₂PO₄ did. D) NaCl, KCl rinsing increased migrated cells by transwell migration assay. One-way ANOVA vs. 0% NaCl, scale bar 100 μm. E) Gene expression of COL1, Fn, FAK with and without NaCl, KCl, NaH₂PO₄ and KH₂PO₄ rinsing by RT-PCR.