Table 1. Primers used in RT-PCR.
Primer | Sequence ID | Sequence (Forward and Reverse 5’-3’) | Base pairs | Cycles |
---|---|---|---|---|
COL1 | NM_000088.3 | F: GCA AAG AAG GCG GCA AA | 500 | 22 |
R: CTC ACC ACg ATC ACC ACT CT | ||||
FAK | NM_004967.3 | F: CAA TCC CAC ACA TCT TGC TGA | 186 | 38 |
R: AGC CGG CAG TAC CCA TCT ATT | ||||
Fn | NM_002026.2 | F: GGA TCA CTT ACG GAG AAA CAG | 401 | 28 |
R: GAC ACT AAC CAC ATA CTC CAC | ||||
GAPDH | NM_002046.3 | F: TgA Agg TCg gAg TCA ACg gAT | 396 | 22 |
R: TCA CAC CCA TgA CgA ACA Tgg |