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. 2016 Jul 21;11(7):e0157391. doi: 10.1371/journal.pone.0157391

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© 2016 Zambonelli et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Panel A: SDS-PAGE conducted under reduced and non-reduced conditions. Panel B: SEC-HPLC showing non-reduced (upper panel) and reduced (lower panel) conditions. Results confirm the presence of the TV1.C gp120 dimeric form; SEC-HPLC, under reducing, but otherwise native conditions, showed that the reduced gp120 monomer and dimer form a homogenous population. Due to the very low amount of dimeric gp120 protein for 1086.C, comparison of reduced and non-reduced conditions is not shown.