Fig 3. Relative levels of miR-223 expression in OSCC cell lines and tissues.

(A) OSCC cell lines: Relative levels of miR-223 expression were evaluated using the equation–log₁₀ 1/2^-⊿⊿Ct in which ΔΔCt = ΔCt_{cell line}^―ΔCt_{normal.tissue.} The ΔCt value of miR-223 in each sample was calculated by normalization with endogenous control RNU6B (ΔCt = Ct_miRNA- Ct_U6RNA). (B) OSCC tissues: The upper panel shows the relative levels of miR-223 expression that were evaluated using the equation 2^―ΔΔCt when 2^―ΔΔCt ≥1 or -1/2^⊿⊿Ct when 2^―ΔΔCt < 1 in which ΔΔCt = ΔCt_{OSCC tissue}^―ΔCt_{normal tissue}, and ΔCt = Ct_miRNA- Ct_U6RNA. The lower panel shows the characteristics of patients from whom cancerous and adjacent non-cancerous tissues were tested. TNM: tumor node metastasis staging system.