Table 2. The value for calculation of fetal DNA concentration.

%chrY_FF (median)		%chrY_AM (median)		%chrY_MF				Fetal DNA concentration region
Original method	Optimized method	Original method	Optimized method	Minimum in original method	Maximum in optimized method	Minimum in original method	Maximum in optimized method	Original method	Optimized method
0. 0000422	0.00000302	0.00173	0.000374	0.000111	0.00170	0.0000727	0.000315	4.1% ~ 19.69%	4.1% ~ 18.38%
The %chrY_MF value when assumption fetal DNA concentration is 1%
Original method		F = 1%	%chrY_FF (median) = 0. 0000422		%chrY_AM (median) = 0.00173		%chrY_MF = 0.0000591
Optimized method		F = 1%	%chrY_FF (median) = 0.00000302		%chrY_AM (median) = 0.000374		%chrY_MF = 0.00002