| Strains |
|
|
|
Pseudomonas protegens Pf-5 |
Wild-type |
Howell and Stipanovic (1979) |
| ΔpchH
|
P. protegens Pf-5 ΔpchH
|
This study |
| ΔfetF
|
P. protegens Pf-5 ΔfetF
|
This study |
| Δ3504 |
P. protegens Pf-5 ΔPFL_3504 |
This study |
|
Escherichia coli S17-1 |
recA thi pro, tra genes (from plasmid RP4) integrated into chromosome |
Simon et al. (1983) |
|
Escherichia coli TOP10 |
F- mcrA (mrr-hsdRMS-mcrBC) 80lacZM15 lacX74 recA1 ara139 (ara-leu)7697 galU galK rpsL (StrR) endA1 nupG
|
Invitrogen |
| Plasmids |
|
|
| pGEM-T Easy |
TA cloning plasmid vector, ApR
|
Promega |
| pEX18Tc |
Broad-host-range suicide plasmid vector; TcR
|
Hoang et al. (1998) |
| pEX18Tc-3495NF |
1.1 kb PCR fragment containing truncated (from nt 254 to nt 1464) pchH gene cloned into HindIII-BamHI sites of pEX18Tc; used to construct ΔpchH strain |
This study |
| pEX18Tc-3503NF |
1.1 kb PCR fragment containing truncated (from nt 121 to nt 920) fetF gene cloned into HindIII-BamHI sites of pEX18Tc; used to construct ΔfetF strain |
This study |
| pEX18Tc-3504NF |
1.1 kb PCR fragment containing truncated (from nt 82 to nt 1011) PFL_3504 gene cloned into HindIII-BamHI sites of pEX18Tc; used to construct Δ3504 strain |
This study |
| pBBR1-MCS2 |
5.1-kb broad-host range plasmid; KmR
lacZ
|
Kovach et al. (1995) |
| pBBR1-pchH |
Two distinct PCR products of pchH and native promoter region upstream of pchD separately cloned into pBBR1-MCS2 at HindIII-EcoRI and EcoRI-BamHI sites respectively |
This study |
| pBBR1-fetF |
PCR product containing fetF gene with downstream terminator region was cloned into HindIII-EcoRI site of pBBR1-MCS2 while a PCR product containing the native promoter region upstream of fetA was cloned into the same pBBR1-MCS2 vector at EcoRI-BamHI site |
This study |
| pBBR1-3504 |
PCR product containing contiguous PFL_3504 gene with native promoter and terminator regions cloned into pBBR1-MCS2 at HindIII-EcoRI sites. |
This study |
