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. 2016 Jul 21;11(7):e0159646. doi: 10.1371/journal.pone.0159646

Fig 7. LIC2 biochemically interacts with key SAC proteins at metaphase.

Fig 7

A) Western blots of affinity purified mitotic LIC2 (LIC2-MTAP) probing for interaction of SAC proteins Mad1, Zw10 and BubR1 (right panel), and with the empty tag control (left panel). ß-actin was used as the loading control. Control indicates empty tag (MTAP vector alone) pulldown, LIC2 indicates LIC2-MTAP (see Materials and Methods). B) Upper panels: Immunoblots probing for the pulldown of dynein subunits DHC (dynein heavy chain) and IC-74 (dynein intermediate chain) upon affinity purification of the empty MTAP tag or LIC2-MTAP. Lower panels show successful pulldown of the respective bait proteins as positive controls for the affinity purification reaction. IN = input, FT = flow through, WS = wash, AP = affinity pulldown precipitate.