Table 1. Selection of tool SSOs with documented in vivo hepatotoxicity (ALT levels after 2 week treatment in mice with 5 x 15 mg/kg tail vein injection) that were used for the validation of in vitro hepatotoxicity assays.

SSO	ALT [U/L]	Sequence
32	64	5'- mCS AS AS aS gS gS aS aS aS cS aS cS aSmCS AS T -3'
33	59	5'- mCS AS AS aS tS gS cS tS gS aS aS aS cS TS AS T -3'
35	67	5'- mCS TSmCS aS aS cS aS tS cS aS aS gS cS AS GS T -3'
36	1889	5'- ASmCS TS gS cS tS tS tS cS cS aS cS tSmCS TS G -3'
37	2368	5'- GSmCSmCS tS cS cS cS aS gS tS tS cS cS TS TS T -3'
43	1890	5'- GS AS TS gS cS cS tS cS cS cS aS GS TS T -3'
47	ND	5'- GS AS cS aS tS tS gS cS cS tSmCS TS A -3'

ND: no ALT levels were determined, since group had to be sacrificed early due to severe toxicity. Small letters: nucleotides; s: phosphorothioate backbone; Capital letters: beta-oxy LNA, ^mC: methylated cytosine.