Table 3. Summary of ribosomal protein alterations in the S. aureus cytoplasmic extracts following growth under the various sets of environmental conditions in comparison with the reference control samples.
Growth conditions | Ribosomal proteins identified which did not alter in cytoplasmic concentrations whilst the majority of the ribosomal proteins were either up-regulated or down-regulated in cytoplasmic composition relative to the control | Ribosomal proteins that had significant increases or decreases relative to the control in contrast to the responses for the majority of ribosomal proteins |
---|---|---|
(B) Centroid: pH7; 37°C; 2.5% NaCl; n = 4 | S18: S19; L3; L7/L12; L10; L22; L23; L32 | |
Majority of ribosomal proteins increased | ||
(C) pH6; 35°C; 0%NaC; n = 4 | Up-regulated: L10 | |
No majority ribosomal protein response | Down-regulated: L9; L15; S6 | |
(D) pH8; 35°C; 0%NaCl; n = 4 | Up-regulated: | |
No majority ribosomal protein response | Down-regulated: L9; L33 | |
(E) pH6; 35°C; 5%NaCl; n = 4 | S6; S17; S19; S20; L5; L28; L30; L31 Type B; L32; L33 | Up-regulated: L7/L12; L10; L29 |
Majority of ribosomal proteins decreased | Down-regulated: | |
(F) pH8; 35°C; 5%NaCl; n = 4 | S18; S19; L10; L23; L28 L32 | |
Majority of ribosomal proteins increased |
Control cultures were grown under ideal conditions at pH7 and 37°C with no added NaCl; (B) centroid cultures grown at pH 7 and 37°C with 2.5% NaCl; (C) 35°C and pH6 with no added NaCl; (D) 35°C and pH8 with no added NaCl; (E) 35°C and pH6 with 5% NaCl added; (F) 35°C and pH8 with 5% NaCl