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. 2016 May 20;12:1744806916649191. doi: 10.1177/1744806916649191

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Increased percentage of menthol sensitive neurons in CB1R−/− DRG cultures. Primary cultures were generated from lumbar DRG isolated from CB1R+/+ (N = 4 mice, 11 coverslips) and CB1R−/− (N = 5 mice, 10 coverslips), loaded with FURA-2 and stimulated with the TRPM8 agonist menthol. (a) Pie-graphs depicting the percentage of menthol sensitive (MS) and menthol-insensitive (MI) neurons per genotype with the number of neurons imaged placed in parentheses; CB1R−/− had a significantly higher percentage of menthol sensitive neurons, p < 0.0001, Fisher’s exact test. (b) Average amplitude of peak menthol responses in CB1R+/+ and CB1R−/− cultures. Data are presented as mean amplitude of the ΔF/F_o ± SEM. ** p < 0.05, Student’s t-test. Scatter plot of neuronal diameters versus peak menthol response; each point represents a single neuron from (c) CB1R+/+ and (d) CB1R−/− cultures. Relative frequency of (e) ‘MS’ and (f) ‘MI’ DRG neurons in CB1R+/+ and CB1R−/− according to neuron diameter (in µm). (e) Frequency distribution (in percentage) of all DRG neurons in culture per diameter and per genotype; bin centers set at 5 µm.