Figure 6.

Local effects of inhibitors on SNIt-induced mechanical hypersensitivity. (a, c, e) PKC inhibitor (GF 109203X) and (b, d, f) mTOR complex 1 inhibitor (rapamycin) were used in the rats after SNIt and Sham by intraplantar injections for the behavioral assessments of (a, b) thermal hyperalgesia, (c, d) mechanical hyperalgesia, and (e, f) mechanical allodynia. The withdrawal responses in surgical side were represented as mean ± SD in withdrawal latency, pin-prick duration, and mechanical threshold following each PIH. Based on the concentrations of inhibitors, the rats after SNIt were separated into three groups (n = 5 for each group) for each treatment. The maximum concentration was used in the rats after Sham (n = 5). Student’s t test was applied to examine the differences against vehicle injections after SNIt at each time point. * p < 0.05 indicated a significant difference. One-way repeated measures ANOVA was also used to analyze within-group differences following the Dunnett’s multiple comparison tests.