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. 2016 Jun 13;12:1744806916656382. doi: 10.1177/1744806916656382

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — GJG inhibited the expression of Iba1, a microglial activation marker, in the ipsilateral dorsal horn. (a) Immunohistochemical analysis of Iba1 from day 1 to day 7 post-operation. Confocal images were taken at 40 × magnification. Magenta fluorescence indicates Iba1-positive cells. Scale bar = 100 µm. (b) Results of Western blotting analysis of Iba1 from day 1 to day 7 post-operation. GAPDH is shown as a loading control. (c) Quantification of Western blotting analysis of Iba1 in Sham, GJG-treated Sham, CCI, and GJG-treated CCI groups on day 3 post-operation. The left blot was densitometrically analyzed, and the ratio of Iba1 immunoreactivity to GAPDH immunoreactivity was determined. Each data point represents the mean ± SD. n = 3. Data were analyzed using Dunnett’s test (*p < 0.05 indicates a significant difference between the indicated groups).