Skip to main content
. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: ACS Appl Mater Interfaces. 2016 Jul 11;8(28):17775–17783. doi: 10.1021/acsami.6b03245

Figure 7.

Figure 7

The efficacy of doxorubicin (DOX)-loaded Alg-MA sub-microspheres as chemotherapeutic delivery vehicles was assessed using a MTT-based assay, to quantify cell proliferation over a 5-day period. A549 activity was recorded as mitochondrial activity and normalized to non-modified cell controls. Various formulations and concentrations (10-100 μg/mL) of sub-microspheres were assessed: green photo-crosslinked (Green), green + Ca2+ dual-crosslinked (Green+C), UV photo-crosslinked (UV), UV + Ca2+ dual-crosslinked (UV+C). DOX was added exogenously (Free DOX) to the cell culture medium at various concentrations to test the effect of intracellular versus extracellular DOX delivery. (A) Effect of Alg-MA sub-microsphere concentration for each crosslinking type on A549 mitochondrial activity; (B) Effect of ‘free dox’ concentration on A549 mitochondrial activity on days 1, 3, and 5; (C) Effect of DOX concentration encapsulated within Alg-MA sub-microspheres on A549 mitochondrial activity on days 1, 3, and 5.