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. Author manuscript; available in PMC: 2016 Nov 12.
Published in final edited form as: Genes Immun. 2016 May 12;17(5):283–297. doi: 10.1038/gene.2016.19

Figure 3. H3K4me2 dominance at rest promotes transcriptional upregulation 1 day after activation of naïve CD4 T cells.

Figure 3

A) Mean increase in RNA expression (Log2 Fold Change) 1 day after activation of naïve CD4 T cells is plotted for genes with H3K4me2 peaks alone, H3K4me3 peaks alone, or both H3K4me2 and H3K4me3 peaks. Error bars represent standard error of the mean. Reported p-values are from Tukey's HSD post-hoc analysis following one-way ANOVA (*****p<1×10−6) B) Mean increase in RNA expression 1 day after activation is plotted for genes with a continuum of H3K4me2/H3K4me3 at rest in naïve cells. Error bars represent standard error of the mean. Reported p-values are from Tukey's HSD post-hoc analysis following one-way ANOVA (****p<1×10−5). C) Genome viewer landscape of H3K4me2 (upper panel) and H3K4me3 (lower panel) in the promoter of IL2. A called peak for H3K4me2 is denoted by the arrow head. TSS = Transcriptional Start Site. D) ChIP-Seq FPKM for H3K4me2 and H3K4me3 in the IL2 promoter of resting naïve CD4 T cells 1 kb on either side of the TSS. E) The RNA increase in IL2 transcript 1 day after activation of naïve CD4 T cells as measured by RNA-Seq.