Fig. 4.

Pharmacological modulation of TGF-α promoter activity occurs via NF-κB. (A) Astrocytes were transfected overnight with WT or TM TGF-α luciferase plasmid constructs, followed by treatment with 250 μM of dbcAMP and determination of TGF-α promoter activity by luciferase assay. (B) After overnight transfection with WT or TM TGF-α luciferase plasmids, cells were treated with 100 nM of DX for 24 h. TGF-α promoter activity was determined by luciferase assay. (*p<0.05, ***p<0.001, ###p<0.001; significantly different from the control or indicated groups; N.S., not significant; two-way ANOVA followed by Tukey’s post hoc test; N=3).