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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Neurobiol. 2016 Jan 21;54(2):964–976. doi: 10.1007/s12035-016-9705-9

Fig. 7.

Fig. 7

YY1 negatively regulates TGF-α promoter activity. (A) Astrocytes were transfected overnight with 0.5 μg of WT or YY1 binding site mutant (YY1m, −800 site of TGF-α promoter) TGF-α luciferase plasmid, followed by the determination of TGF-α promoter activity. (B) Cells were co-transfected overnight with 0.5 μg of TGF-α luciferase plasmid and 0.1 μg of either EV pcDNA or YY1, followed by the measurement of TGF-α promoter activity. (C) Cells were transfected overnight with 2 μg of EV pcDNA or YY1, followed by TGF-α mRNA analysis with qPCR. The overexpression of YY1 was confirmed by western blotting. (**p<0.01, ***p<0.001, #p<0.05; significantly different from the control or indicated groups; Student’s t-test or two-way ANOVA followed by Tukey’s post hoc test; N=3).