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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Neurobiol. 2016 Jan 21;54(2):964–976. doi: 10.1007/s12035-016-9705-9

Fig. 8.

Fig. 8

Mn decreases TGF-α expression via activation of YY1. (A) After transfection with the YY1 promoter construct overnight, astrocytes were treated for 3 h with Mn (250 μM), followed by luciferase assay. (B) Cells were treated with Mn (250 μM) for 3 h, followed by qPCR to measure YY1 mRNA levels. (C and D) Cells were treated for 6 h with Mn (250 μM), followed by measurement of TGF-α mRNA levels with qPCR (C) and TGF-α protein levels with western blot (D). (E) After transfection with the TGF-α promoter constructs (WT and YY1 mutant) overnight, astrocytes were treated for 6 h with vehicle or Mn (250 μM), followed by luciferase assay. (*p<0.05, **p<0.01, #p<0.05, ##p<0.01; significantly different from the control or indicated groups; Student’s t-test or two-way ANOVA followed by Tukey’s post hoc test; N=3).