Figure 3.

NFIB Promotes Metastases and Changes the Metastatic Profile

(A) Percent of animals with and without liver metastasis in each of the four classes.

(B) Quantification of the number of liver metastasis in each class.

(C) Quantification of the percent area of the liver covered by metastatic lesions in each of the four classes. Circles with a shaded upper or lower half indicate animals with kidney and bone metastasis, respectively. Circles marked with a central dot indicate animals with metastasis to both kidney and bone.

(D–F) Representative liver section from the three classes, Mycl (D), Nfib (E), and Nfib/Mycl (F), stained with NCAM to identify NE metastatic lesions.

(G) NE metastasis in the kidney, H&E.

(H) Percent of animals in each class with metastatic lesions outside the liver (bone, kidney).

(I) Number of animals in Mycl and Nfib/Mycl cohort that provided successful culture of NE cells from the blood.

Scale bars in (D)–(F), 200 μm. Scale bar in (G) represents 100 μm. See also Figure S4.