Figure 5.

NFIB Drives Tumor Dedifferentiation and Invasion

(A–C) Early lesions NFIB (A), SYN (B), and CDH1 (C) staining, respectively (Nfib cohort).

(D–F) Advanced lesion NFIB (D), SYN (E), and CDH1 (F) staining, respectively (Mycl cohort).

(G) Whole lung, CDH1 staining (Mycl cohort).

(H and I) Part of the lung indicated with dotted area in (G), CDH1 (H) and NFIB (I), respectively. Invasive sheaths indicated with arrowheads.

(J) Whole lung, CDH1 staining (Nfib cohort).

(K and L) Diagrams of tumor growth and local and distant metastatic dissemination. NE tumor cells are abundantly found in lymph vessels. Without NFIB overexpression, intrapulmonary dissemination and metastatic load in liver is very limited (K). With NFIB overexpression, there is massive intrapulmonary dissemination, large metastatic load in the liver, and metastasis to kidney and bone (L).

Scale bars in (A)–(F) represent 100 μm. Scale bars in (H) and (I) represent 500 μm. See also Figure S6.