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. Author manuscript; available in PMC: 2016 Jul 22.
Published in final edited form as: Methods Mol Biol. 2016;1413:111–133. doi: 10.1007/978-1-4939-3542-0_8

Figure 1. Schematic overview of the in vitro kinetochore assembly process.

Figure 1

Recombinant nucleosome arrays containing CENP-A nucleosomes can recruit functional centromere and kinetochore components when incubated in Xenopus egg extract. Prior attachment of the arrays to magnetic beads allow for easy reisolation of chromatin arrays and any bound complexes for further downstream analysis. Four example experiments utilizing this setup for the analysis of centromere and kinetochore complexes are summarized. These experiments are described in detail in the text.