Figure 1.
Photoactivated localization microscopy (PALM)-point accumulation-for-imaging-in-nanoscale-topography (PAINT), single-molecule tracking during PALM imaging. (A) (Left) Super ecliptic phluorin (SEP)-TM-Bind molecules tracked for at least eight frames super-imposed on molecules of shrPSD-95, accumulated from 5000–20,000 frames. (Right) A typical example of tracked probes superimposed on positions of shrPSD-95 molecules. The first and last localized positions are indicated as filled and open circles, respectively. (B) Mean squared displacement (MSD) over time of connected sub-segments of tracks (subtracks) that are lasted at least 15 frames (n = 156 synaptic and 2907 extrasynaptic tracks/113 PSDs/13 fields/11 cells/3 cultures). (C) Cumulative frequency distributions of Deff for subtracks that are at least eight frames long (n = 654 synaptic and 3349 extrasynaptic tracks/113/13/11/3). ****p < 0.0001.