Table 1.
Gene | Sequence of primers | Length of amplicon (bp) | Tm (°C) |
---|---|---|---|
SOD1 | Forward: 5′-TTGGGCAATGTGACTGCTGACAAA-3′ Reverse: 5′-GGGCGATCCCAATTACACCACAA-3′ |
208 | 79.0 |
SOD2 | Forward: 5′-CTGATTTGGACAAGCAGCAA-3′ Reverse: 5′-CTGGACAAACCTCAGCCCTA-3′ |
199 | 81.6 |
GPX1 | Forward: 5′-AATGTGGCGTCCCTCTGAGGCA-3′ Reverse: 5′-GCTCGTTCATCTGGGTGTAGTCCCG-3′ |
55 | 85.0 |
MGST1 | Forward: 5′-ATTGGCCTCCTGTATTCCTTG-3′ Reverse: 5′-TAATCCCTCTGCTCCCCTCC-3′ |
311 | 80.2 |
GSR | Forward: 5′-AGAAATCATCCGTGGCCATGCA-3′ Reverse: 5′-ACCAACAATGACGCTGCGGC-3′ |
214 | 82.0 |
CAT | Forward: 5′-CCTATCCTGACACTCACCGCCATCG-3′ Reverse: 5′-GGATGCTGTGCTCCAGGGCAGA-3′ |
201 | 82.0 |
β-actin | Forward: 5′-TCACCCACACTGTGCCCATCTACGA-3′ Reverse: 5′-CAGCGGAACCGCTCATTGCCAATGG-3′ |
295 | 85.0 |
bp base pairs, Tm melting temperature