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. 2016 Apr 15;23:14989–14996. doi: 10.1007/s11356-016-6653-x

Table 1.

Characteristic of primers used for real-time QRT-PCR

Gene Sequence of primers Length of amplicon (bp) Tm (°C)
SOD1 Forward: 5′-TTGGGCAATGTGACTGCTGACAAA-3′
Reverse: 5′-GGGCGATCCCAATTACACCACAA-3′
208 79.0
SOD2 Forward: 5′-CTGATTTGGACAAGCAGCAA-3′
Reverse: 5′-CTGGACAAACCTCAGCCCTA-3′
199 81.6
GPX1 Forward: 5′-AATGTGGCGTCCCTCTGAGGCA-3′
Reverse: 5′-GCTCGTTCATCTGGGTGTAGTCCCG-3′
55 85.0
MGST1 Forward: 5′-ATTGGCCTCCTGTATTCCTTG-3′
Reverse: 5′-TAATCCCTCTGCTCCCCTCC-3′
311 80.2
GSR Forward: 5′-AGAAATCATCCGTGGCCATGCA-3′
Reverse: 5′-ACCAACAATGACGCTGCGGC-3′
214 82.0
CAT Forward: 5′-CCTATCCTGACACTCACCGCCATCG-3′
Reverse: 5′-GGATGCTGTGCTCCAGGGCAGA-3′
201 82.0
β-actin Forward: 5′-TCACCCACACTGTGCCCATCTACGA-3′
Reverse: 5′-CAGCGGAACCGCTCATTGCCAATGG-3′
295 85.0

bp base pairs, Tm melting temperature