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. 2016 Jul 21;6:30054. doi: 10.1038/srep30054

Figure 1. I-BAR domains from ABBA can form tubes pointing into Giant Unilamellar lipid Vesicles (GUVs) at high density or bind to existing tubes at lower density.

Figure 1

(A,B) Schematic depiction showing I-BAR binding to tubes pointing inwards into the lumen of a GUV. (C) Incubation of a GUV with 2.3 μM I-BAR. Graph shows radial intensity plot of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. (D) Incubation of a GUV with 290 nM I-BAR. Graph shows radial intensity of TR-DHPE signal (red) and YFP labeled I-BAR signal (green) from a GUV containing a number of inward pointing tubes as shown by the confocal images. Each intensity plotted in (C,D) is the average of all pixels with the same distance to the center of the GUV. The YFP intensities in (C,D) are normalized by the same constant. Membrane composition is DOPC:DOPS:TR-DHPE 59.7:40:0.3.