Table I.
Characterizing Transcriptional Organization
| Gene | Cells expressing gene (%) |
Cells expressing two active alleles (%) |
Mean distance to chromatin boundary (nm) |
Mean radial location (%) |
Standard deviation of radial location distribution |
Clustering factor (inter- cellular) |
|---|---|---|---|---|---|---|
| ACTB | 48 | 21 | 277 | 58 | 19 | 1.02 |
| JUN | 62 | 24 | 140 | 67 | 22 | 1.03 |
| MYC | 17 | 1.7 | — | 62 | 24 | 0.850* |
| CCND1 | 15 | 1.7 | — | 57 | 20 | 0.992 |
| DUSP1 | 28 | 8.8 | 100 | 63 | 24 | 1.09 |
| EGR1 | 67 | 33 | 167 | 62 | 19 | 0.945* |
| ACTG1 | 80 | 41 | 207 | 48 | 18 | 0.998 |
| IL8 | 28 | 6.6 | 162 | 55 | 19 | 1.10* |
| MCL1 | 14 | 2.8 | — | 57 | 15 | 1.09 |
| KLF10 | 78 | 40 | 185 | 58 | 17 | 1.02 |
Summary of five measurements made to characterize transcriptional order. Intranuclear distances between transcription sites are shown in Figure 1H–I. Clustering factor is the mean intercellular distance of cells co-expressing a gene normalized by the mean intercellular distance of every cell (Fig. 3A–B).
*
Clustering factors have statistically significant intercellular distance frequency distributions (Mann-Whitney P < 0.05).