Table II.
Intercellular Spatial Distributions
| Gene | Cells expressing gene (%) |
P-value (Mann- Whitney) |
Clustering factor |
|---|---|---|---|
| MYC | 17 | 0.0046 | 0.850* |
| EGR1 | 67 | 0.014 | 0.945* |
| CCND1 | 15 | 0.055 | 0.992 |
| ACTG1 | 80 | 0.78 | 0.998 |
| ACTB | 48 | 0.81 | 1.02 |
| KLF10 | 78 | 0.96 | 1.02 |
| JUN | 62 | 0.77 | 1.03 |
| DUSP1 | 28 | 0.075 | 1.09 |
| MCL1 | 14 | 0.38 | 1.09 |
| IL8 | 28 | 0.042 | 1.10* |
Expression profiles for 10 genes were interrogated by RNA FISH in DLD-1 human colon adenocarcinoma cells. The clustering factor is the mean intercellular distance of cells co-expressing a gene normalized by the mean intercellular distance of every cell (Fig. 3A–B). The Table is sorted by increasing clustering factor. The clustering factor for each gene is not correlated to expression (Spearman non-parametric correlation test P = 0.63).
*
Clustering factors for genes with significantly different frequency distributions (P < 0.05, Mann-Whitney).