Skip to main content
. Author manuscript; available in PMC: 2016 Jul 22.
Published in final edited form as: Curr Opin Microbiol. 2003 Dec;6(6):614–620. doi: 10.1016/j.mib.2003.10.005

Figure 2.

Figure 2

Ash1 mRNA localization. (a) Cartoon of the Ash1 mRNA core locasome transported on an actin filament. E1, E2a, E2b and E3 zipcodes are recognized by the She1–3p complex, providing the motor activity. (b)Schematic view of the different steps of ASH1 mRNA bud tip localization (see also Table1). 1 – Nuclear events: ASH1 transcription in late anaphase, association of ASH1 mRNA with She2p, interaction with Loc1p. 2 – Early cytoplasmic events: Assembly of the locasome by association of the newly exported ASH1mRNA-She2p RNP with She3p and She1p/Myo4p, recruitment to the actin network and interaction with Khd1p and She4p. 3 – Transport of the locasome to the bud tip. She1p/Myo4 provides the motor activity. Role of She4p as a translocation co-factor. 4 – Anchoring and translation at the bud tip: the mechanism of anchoring is still not understood; nevertheless it involves Khd1p and the nascent Ash1p polypeptide. Puf5p and Scp160p are not represented because their role remains largely unknown. (c) Upper image: phase image of a yeast budding cell in early anaphase. Middle image: fluorescent in situ hybridization (FISH) ofASH1 mRNA using 6 CY3-labeled probes in red and DAPI staining in blue. Lower image: overlay of the two previous images.