Table 1.
Proteins involved in ASH1 mRNA localization.
| Protei n |
Altern ative names |
Function | Role in ASH1 mRNA localization |
Locaso me associ ation |
Subcell ular localiz ation |
Δ phenot ype bud/bu d tip |
References |
|---|---|---|---|---|---|---|---|
| She1 p |
Myo4p | Unconven tional myosin V type motor |
Locasome molecular motor |
Yes | C, bud enriche d |
Abolis hed |
13, 14, 17•, 21, 22. |
| She2 p |
RNA- binding |
Binds ASH1m RNA zipcodes |
Yes | N/C, bud enriche d |
Abolis hed |
13, 18, 19, 20•, 21, 22. | |
| She3 p |
Myosin binding protein |
Molecular link in between She2p and She1p |
Yes | C, bud enriche d |
Abolis hed |
13, 18, 19, 21, 22, 24. |
|
| She4 p |
Dim1p | Endocyto sis Enhance associatio n of Myo3p and Myo5p to the actin network Interacts with She1p |
Enhance association of She1p to the actin network? |
Yes | C, bud enriche d |
nd/nd bud neck |
13, 21, 28, 29. |
| She5 p |
Bni1p, Ppf3p |
Actin filament polymeriz ation at the bud tip |
Polarization and growth of the actin network |
No | C, bud tip |
nd/nd bud neck/b ud enrich ed |
13, 22, 26, 33. |
| Bud growth Establish ment of cell polarity |
Interacts with Bud6p (two- hybrid and synthetic lethality) |
||||||
| Bud6 p |
Aip3p | Actin filament organizati on at the bud tip |
Polarization and growth of the actin network |
No | C, bud tip |
nd/nd bud enrich ed |
26, 27, 34, 35. |
| Bud growth Establish ment of cell polarity |
Interacts with She5p (two- hybrid and synthetic lethality) |
||||||
| Loc1 p |
RNA binding rRNA processin g |
Binds ASH1zi pcodes, general translation defect? |
Yes (nucleu s) |
No | nd/13 | 36, 37. | |
| Khd1 p |
Hek2p | RNA binding, KH domain |
Involved in mRNA bud tip anchoring, translational repression |
Yes | C | 93/40 | 38••, 39. |
| Puf5p | Mpt5p, Htr1p, Uth4p |
Translatio nal regulation mRNA stability |
Involved in mRNA bud tip anchoring, Translational regulation? |
No | nd | 83/22 | 38••, 40. |
| Scp1 60p |
RNA binding, KH domain ER polysome s binding |
Involved in mRNA bud tip anchoring, Translational regulation? |
No | C | 84/23 | 38••, 42. |
C, Cytoplasmic; N, Nuclear; No, nucleolar; deletion phenotype is shown as: percentage of cells showing daughter cell localization of ASH1 mRNA/percentage of cells showing daughter cell bud tip localization ofASH1 mRNA. The wild type strain shows a phenotype of 99/87 [38••].