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. 2016 Apr 21;20(8):1503–1512. doi: 10.1111/jcmm.12838

Figure 2.

Figure 2

The effects of miR‐224 on differentiation of mouse SSCs. (A) Real‐time PCR analysis revealed that miR‐224 mimics and inhibitor are effectively transfected into SSCs. To compare the expression of miR‐224 in different transfection cells, the expression of miR‐224 in negative control cells was set as 1. (B and C) Mouse SSCs were transfected with miR‐224 mimic, miRNA mimic control, miR‐224 inhibitor or miRNA inhibitor control, followed by proliferation assay (B) and EdU incorporation assay (C). (D) Quantification of EdU assay. Data are presented as mean ± SD from three independent experiments. **P < 0.01 compared with the control group. Scale bars = 20 μm. SSC, spermatogonial stem cell.