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The effects of miR‐224 on differentiation marker of SSCs. (A–D) Mouse SSCs were transfected with miR‐224 mimic, miRNA mimic control, miR‐224 inhibitor or miRNA inhibitor control. The expression of GFRα1 and PLZF were measured by RT‐PCR (A and C) and Western blot assays (B and D) respectively. Data are presented as mean ± SD from three independent experiments. **P < 0.01 compared with the control group. SSC, spermatogonial stem cell.
