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Effects of miR‐224 on the expression of GFRα1, PLZF and DMRT1 in mouse testes. (A) qRT‐PCR analysis of miR‐224 expression in male germ cells from recipient mice transplanted with spermatogonial stem cells after pLL3.7‐miR‐224 transfection. (B) Immunohistochemistry showed that GFRα1 and PLZF expression was increased, DMRT1 expression was decreased in mouse testes transplanted with pLL3.7‐miR‐224. Scale bars = 20 μm.
