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. 2016 Mar 28;20(8):1427–1442. doi: 10.1111/jcmm.12832

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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X22 attenuated HFD‐induced myocardial oxidative stress in HFD‐fed rats. (A) Representative images for immunohistochemical staining of 3‐NT accumulation in the formalin‐fixed myocardial tissues (400× magnification). (B) The mRNA expression of the anti‐oxidative markers Nrf‐2 in the myocardial tissues was determined by real‐time qPCR assay. (C) The Western blot analysis for the protein expression of Nrf‐2 in the myocardial tissues. (D) Representative images for immunohistochemical staining of Nrf2 and DAPI in the formalin‐fixed myocardial tissues (200× magnification). (E–G) The mRNA expression of the anti‐oxidative genes HO‐1, GCLC and GCLM in the heart tissues was determined by real‐time qPCR assay (*, versus HFD group; ^#, versus Ctrl group; * and ^# P < 0.05; ** and ^## P < 0.01; n = 7 per group).