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Loss of the T gene and Foxa2 in Lmbrd1 −/−‐embryos. (A–D) Absence of gastrulation in Lmbrd1 −/−‐embryos demonstrated by in situ hybridization studies. In Lmbrd1 +/+‐embryos T is detectable in the primitive streak (A), whereas it is absent in Lmbrd1 −/−‐embryos (B). Foxa2 is expressed in the endoderm of Lmbrd1 +/+‐embryos (C) whereas Lmbrd1 −/−‐embryos show no Foxa2 expression (D). Scale bars represent 100 μm. Representative embryos are shown (n = 5).
