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. 2016 Apr 21;20(8):1491–1502. doi: 10.1111/jcmm.12837

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of berberine on the expression of EP1 in DN rat kidneys and cultured GMCs. (A) Representative images and quantification of protein by western blot for the EP1 receptor in DN rat kidneys are shown. β‐actin served as an internal control. (B) Cells were treated in the presence or absence of berberine (60 μM) for 15 min. EP1 expression was detected by western blotting with anti‐EP1 antibody assay, and the intensity of each band was normalized to β‐actin. Values are the mean ± S.D. of five experiments. **P < 0.01 versus NC rats; ^# ^# P < 0.01 compared with DNC rats.