Table 1.
Trypan blue dyeing determined the cells’ vigor
| BBR | Cell mortality (%) | |
|---|---|---|
| BBR (24 hrs) | BBR (48 hrs) | |
| 5 (μM) | 2.0 ± 0.10 | 2.5 ± 0.11 |
| 10 (μM) | 2.0 ± 0.12 | 3.1 ± 0.10 |
| 30 (μM) | 3.1 ± 0.28 | 3.2 ± 0.23 |
| 60 (μM) | 4.6 ± 0.25 | 4.9 ± 0.21 |
| 90 (μM) | 9.6 ± 0.10 | 16.0 ± 0.41 |
| 120 (μM) | 15 ± 0.14 | 19.5 ± 0.25 |
| 240 (μM) | 17.2 ± 0.21 | 16.3 ± 0.32 |
Cells were treated with different concentrations of berberine and the inhibitory ratio was calculated by the following formula: Cell mortality (%) = (the dead cell number/the total number) × 100%. Values are mean ± S.D., n = 3.