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. 2016 May 16;291(30):15428–15446. doi: 10.1074/jbc.M116.719633

FIGURE 2.

FIGURE 2.

Hmgb2 and Ctcf coregulate each other. A, hmgb2 overexpression (hmgb2 virus for 24 h) or knockdown (hmgb2 siRNA for 72 h) was carried out in NRVMs and confirmed by Western blotting. B, qPCR revealed down-regulation of ctcf with hmgb2 overexpression, and up-regulation of ctcf with hmgb2 knockdown (n = 3, * indicates p value <0.05 (t test), error = standard deviation). hmgb2 knockdown caused no change in histone H1. C, similarly, ctcf knockdown caused an up-regulation of Hmgb2 at the mRNA and protein level, whereas ctcf overexpression down-regulated hmgb2 (n = 5 overexpression, n = 3 knockdown, ** indicates p value <0.01, error = standard deviation). hmgb2 overexpression caused up-regulation of ctcf. D, immunolabeling for Hmgb2 and Ctcf in NRVMs confirmed an increase in Hmgb2 abundance after Ctcf knockdown. Bar, 5 μm. All Western blotting assays and qPCR experiments are one representative experiment of at least three. Images are one representative of approximately n = 100.