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DSF profilin proline screening. Amb a 8, Art v 4, Bet v 2, and their His-tagged counterparts were screened against 10.0 mm l-proline, Pro6, Pro10, or Pro14 to determine change in protein melting temperature (Tm). The control was profilin stability in gel filtration buffer (10 mm Tris, pH 7.4, 150 mm NaCl). Error bars, S.D.
