FIGURE 10.

Effects of replacing the residues of rPyAly with cysteine on its temperature dependence. Enzyme reactions were conducted in a solution containing 10 mm sodium phosphate (pH 8.0), 0.1 m NaCl, 0.1 mg/ml BSA, 0.25% (w/v) polyM, and 0.005 mg/ml rPyAly (A), rPyAlyG79C (B), rPyAlyD230C (C), or rPyAlyG79C/D230C (D) for 15 min at the indicated temperatures. Relative activity at 100% was equivalent to 1,820 units/mg (A), 1,740 units/mg (B), 1,710 units/mg (C), and 1,780 units/mg (D). All assays were repeated three times, and the data are shown as mean ± S.D.