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Evaluation of an alginate lyase activity of rNitAly. Relative viscosity (closed circles) and absorbance at 235 nm (open circles) were measured in a mixture of 10 mm sodium phosphate (pH 6.0), 1 m NaCl, 0.1 mg/ml BSA, 1% (w/v) sodium alginate, and 0.005 mg/ml rNitAly at 30 °C at the indicated time points.
