TABLE 1.

Primers used in this study

Introduced restriction sites are underlined. Mutated sequences are double underlined. F indicates forward; R indicates reverse.

Primer	Sequence
For nitaly cloning
Nit-F	5′-ATGCACCAACTAAAAGTTTTG-3′
Nit-R	5′-CTATTTCATTGTTAATGAATC-3′
For rNitAly expression
Nit-BamF	5′-GCGGATCCCACGATGCGCCCTACGCTAT-3′
Nit-XbaR	5′-CGTCTAGACTATTTCATTGTTAATGAAT-3′
For rNitAlyC80A, rNitAlyC232A, or rNitAlyC80A/C232A expression
Nit-C80A-F	5′-ACCTTTTTCATGGCCGGGAAAAAACAT-3′
Nit-C80A-R	5′-ATGTTTTTTCCCGGCCATGAAAAAGGT-3′
Nit-C232A-F	5′-CAAGGAGACGGAGCCGCAAAAGTTTT-3′
Nit-C232A-R	5′-AAAACTTTTGCGGCTCCGTCTCCTTG-3′
For rPyAlyG79C, rPyAlyD230C, or rPyAlyG79C/D230C expression
Py-G79C-F	5′-GTGTTTGTCATGTGTGGCGACTCAC-3′
Py-G79C-R	5′-GTGAGTCGCCACACATGACAAACAC-3′
Py-D230C-F	5′-GAGGGCAGCCCCTGCGCGAGGGTAGTG-3′
Py-D230C-R	5′-CACTACCCTCGCGCAGGGGCTGCCCTC-3′