TABLE 2.

Effects of monovalent cations on the alginate lyase activity of NitAly

	Relative activitya (%)
	0.1 m	0.5 m	1.0 m
NaCl	9.2 ± 0.5	74.2 ± 2.1	100 ± 4.3b
KCl	8.3 ± 0.4	71.6 ± 1.8	113 ± 6.1
LiCl	10.3 ± 0.4	77.3 ± 3.2	101 ± 3.0
CsCl	8.7 ± 0.3	72.4 ± 1.2	114 ± 7.4

^a Enzyme reactions were conducted in a solution containing 10 mm sodium phosphate (pH 6.0), 0.1 mg/ml BSA, 0.005 mg/ml rNitAly, 0.5% (w/v) polyM, and the indicated concentration of monovalent cation at 50 °C for 15 min.

^b Relative activity at 100% was equivalent to 1,350 units/mg.